mouse c peptide elisa kit  (ALPCO)

Journal: bioRxiv

Article Title: Loss of Exocytosis Protein DOC2B is an Early Event in Type 1 Diabetes Development

doi: 10.64898/2025.12.28.696610

Figure Lengend Snippet: Plasma from 7-week-old female nonobese diabetes (NOD) and age-, gender, and MHC-matched nonobese diabetes resistant (NOR) mice was evaluated by quantitative immunoblotting for DOC2B protein content. (A) A r epresentative immunoblot is shown from n =10 mice per group. Dashed vertical lines indicate splicing of lanes from within the same gel exposure. Ponceau S served as loading control (37-75 kDa range). (B) Densitometry analysis of the ratio of plasma DOC2B levels (normalized to Ponceau S) in the 7-week-old female NOR versus NOD. (C) Random fasting blood glucose levels were measured in NOR versus NOD mice at 7 weeks of age. (D) Plasma C-peptide levels were assessed using an enzyme-linked immunosorbent assay (ELISA). Data are represented as mean ± SEM; n =10 mice per group; * P <0.05 and n.s.-not significant using one-tailed, unpaired, Student’s t -test for (B), and two-tailed, unpaired, Student’s t -test for [C, D]. (E) DOC2B (magenta), without or with blocking peptide (BP), and insulin (green) immunofluorescence staining; (F) bar graph quantification of DOC2B fluorescence intensity in insulin-positive cells in mouse pancreata, representative of n =4 mice per group. Bar= 10 µm. Merge image contains DOC2B (magenta), insulin (green) and DAPI (blue), nuclear staining. Data represent the mean ± SEM. * P <0.05 by one-tailed, unpaired, Student’s t -test. (G) Quantification of the insulitis scores from hematoxylin-insulin-counterstained pancreatic tissue sections of 7-week-old female NOD and NOR mice, as described in Supplemental Materials and Methods. Data are represented as average mean ± SEM, n =4 mice per group.

Article Snippet: Following plasma separation, C-peptide levels were measured using a mouse C-peptide ELISA kit purchased from ALPCO (Salem, NH, USA, cat# 80-cptms-e01), according to the manufacturer’s instruction.

Techniques: Clinical Proteomics, Western Blot, Control, Enzyme-linked Immunosorbent Assay, One-tailed Test, Two Tailed Test, Blocking Assay, Immunofluorescence, Staining, Fluorescence

Journal: Science Advances

Article Title: A wearable device for continuous immunoassay-based monitoring of C-peptide in interstitial fluid

doi: 10.1126/sciadv.adw2182

Figure Lengend Snippet: ( A ) The ratio of C-peptide concentrations between blood and ISF in mice ( n = 10). ( B ) The comparison for mice C-peptide between the app’s predictions and standard ELISA results. ( C ) C-peptide concentration in ISF and blood glucose for mice with type 1 diabetes, type 2 diabetes, and normal mice ( n = 8). ( D ) The ratio of C-peptide concentrations between blood and ISF in rabbits ( n = 16). ( E ) The comparison for rabbit C-peptide between the app’s predictions and standard ELISA results. ( F ) C-peptide concentrations in rabbit ISF at 0, 30, 60, 120, and 180 min using the CIM device.

Article Snippet: A mouse C-peptide ELISA kit was from Elabscience (TX, USA).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Concentration Assay